Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Analysis of mitochondrial subunit assembly into respiratory chain complexes using Blue Native polyacrylamide gel electrophoresis.

The mitochondrial respiratory chain consists of multi-subunit protein complexes embedded in the inner membrane. Although the majority of subunits are encoded by nuclear genes and are imported into mitochondria, 13 subunits in humans are encoded by mitochondrial DNA. The coordinated assembly of subunits encoded from two genomes is a poorly understood process, with assembly pathway defects being ...

2-D gel densitometer for high-contrast and selective imaging of chlorophyll-containing protein complexes separated by non-denaturing polyacrylamide gel electrophoresis.

In this work, we present a home-made two-dimensional (2-D) CCD imaging system for the monochromatic densitometry of plane gels and its application to the imaging and densitometry of chlorophyll (Chl)-containing proteins separated by non-denaturing polyacrylamide gel electrophoresis. The monochromatic imaging of separated green bands at the wavelengths corresponding to their absorption band incr...

Lipoprotein X: BIOCHEMICAL PREDICTORS AND DETECTION BY NON-DENATURING POLYACRYLAMIDE GRADIENT GEL ELECTROPHORESIS

Characterization of goat milk protein and comparison of milk proteins using polyacrylamide gel electrophoresis

Goat milk has been a rich source of proteins. The distinctive composition of goat milk, combined with its nutritional value, is linked to the liberation of protein fragments during digestion or technological processing, which are able to perform various biological activities. This has gain more attention due to its broad applications in the food, drug, and various other industries. Thus, here w...

Polyacrylamide Gel Electrophoresis

Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...